Nephroprotective effect of Astaxanthin against radiotherapy via TAS, TOS (biochemical), TNF-α, CASPASE-3 (immunohistochemical), SIRT-1, P53 (molecular) pathway in rats

Aim: To evaluate the effects of Astaxanthin (ATX), known for its antioxidant properties, on kidneys rats given radiation by biochemical measuring total oxidant level (TOS), (TAS), immunohistochemically Cas3 (Cysteine Aspartate Specific ProteASEs), TNF-α (Tumor necrosis factor-alpha), and molecularly P53, SIRT (Sirtuin -1) pathways.
 
 Materials Methods: The were divided into 4 groups (8 per group): control, radiotherapy (RT), RT+ATX, ATX. ATX was to at mg/kg 7 days. We evaluated effect in rats’ damaged RT comparing all with TAS, TOS, Cas 3, TNF-α, SIRT-1, P53.
 Results: TAS levels similar among RT, groups. TOS significantly lower group compared Control, RT+ATX Histopathologically marked hyperemia some kidneys, small hemorrhages observed group. In addition, glomerular sclerosis also detected this With ATX, we significant improvement Immunohistochemically revealed increased expressions, tubular cells expressions treatment decreased expression No both control There no difference between P53 values.
 Conclusion: that it is a carotenoid may benefit recovery kidney damage after radiation, has positive oxidative stress.